Coding

Part:BBa_K4133009:Design

Designed by: Luis Alejandro Chaves Martinez   Group: iGEM22_Costa_Rica   (2022-10-04)

Repressor protein CtsR_Lc (Lactococcus lactis) for Lactobacillus casei


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was directly obtained from the Lactococcus lactis ctsR gene (GenBank Accession: AJ249133.1). The codon optimization of the part for its expression in L. casei was made using the GenSmart™ Codon Optimization tool from GenScript, adding the RFC10 restriction sites as undesired in the sequence.


Source

This part was de-novo synthesized


References